A comparison between acetylcholine-like action potentials and square depolarizing pulses in triggering calcium entry and exocytosis in bovine chromaffin cells.
J Mol Neurosci
; 30(1-2): 57-8, 2006.
Article
in En
| MEDLINE
| ID: mdl-17192626
ABSTRACT
Depending on experimental conditions, cell model, and pattern and type of depolarizing stimuli, the relationship between calcium entry ([Ca2+]c) and the release of neurotransmitters and hormones varies from exponential (power of 3-4) to near linear (power of 1.5) or linear function. Here, we present a study using the more physiological stimulation pattern based on acetylcholine (ACh)-like action potentials, in voltage-clamped bovine chromaffin cells, with the perforated-patch configuration of the patch-clamp technique and 2 mM extracellular calcium. Trains of ACh-like action potentials or square depolarizing pulses of increasing length were applied, and calcium currents (ICa), total calcium entry (QCa), and exocytosis (DeltaCm) measured.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Action Potentials
/
Acetylcholine
/
Calcium
/
Chromaffin Cells
/
Exocytosis
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Mol Neurosci
Journal subject:
BIOLOGIA MOLECULAR
/
NEUROLOGIA
Year:
2006
Type:
Article
Affiliation country:
Spain