[Apoptosis and gene FasL expression induced by carbon disulfide in rat sertoli cells].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 24(12): 730-3, 2006 Dec.
Article
in Zh
| MEDLINE
| ID: mdl-17241545
ABSTRACT
OBJECTIVE:
To study apoptosis and gene FasL expression induced by carbon disulfide in sertoli cells of male rats.METHODS:
Sertoli cells were exposed to different concentrations of CS(2) (0, 0.36, 0.72, 1.44 micromol/ml) for 24 hours. Survival rate, apoptosis rate, expression level of gene FasL were measured using MTT, FCM, and RT-PCR methods respectively.RESULTS:
Sertoli cell survival rate decreased as the concentration of CS(2) increased. The survival rate (73.34% +/- 1.39%) was significantly lower than the control group (99.98% +/- 5.48%) when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Apoptosis rate increased as the CS(2) concentration increased. Apoptosis rate (7.93% +/- 0.43%) was significantly higher when the concentration of CS(2) > or = 1.44 micromol/ml (P < 0.05). Expression level of the FasL significantly increased as the concentrations of CS(2) (P < 0.05).CONCLUSION:
CS(2) is cytotoxic to sertoli cells. It could cause apoptosis of sertoli cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sertoli Cells
/
Carbon Disulfide
/
Apoptosis
/
Fas Ligand Protein
Limits:
Animals
Language:
Zh
Journal:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Journal subject:
MEDICINA OCUPACIONAL
Year:
2006
Type:
Article
Affiliation country:
China