In situ hybridization for Lawsonia intracellularis--specific 16s rRNA sequence in paraffin-embedded tissue using a digoxigenin-labeled oligonucleotide probe.
J Vet Diagn Invest
; 19(3): 282-5, 2007 May.
Article
in En
| MEDLINE
| ID: mdl-17459858
ABSTRACT
An in situ hybridization (ISH) procedure with a digoxigenin-labeled oligonucleotide probe for detection of Lawsonia intracellularis in paraffin-embedded tissue is described. This technique recognized 71% of PCR-positive cases and was thus superior to Warthin-Starry silver stain, which only detected 41%. The presented ISH is of comparable sensitivity to previously published immunohistochemical assays and is recommended for laboratories wishing to diagnose L. intracellularis infections in tissue sections but without access to antibodies.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine Diseases
/
RNA, Ribosomal, 16S
/
Oligonucleotide Probes
/
In Situ Hybridization
/
Lawsonia Bacteria
/
Desulfovibrionaceae Infections
/
Intestinal Diseases
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Vet Diagn Invest
Journal subject:
MEDICINA VETERINARIA
Year:
2007
Type:
Article
Affiliation country:
Austria