[An observation of the effects of the truncated septin2 on mouse epidermal cell and fibroblast].
Zhonghua Zheng Xing Wai Ke Za Zhi
; 23(2): 147-50, 2007 Mar.
Article
in Zh
| MEDLINE
| ID: mdl-17554884
ABSTRACT
OBJECTIVE:
To construct eukaryotic expression vector of the truncated septin2 and investigate the influence on the cultured mouse epidermal cell and fibroblast in vitro exerted by the transgenic expression product.METHODS:
The short splicing fragment was obtained by amplifying the reverse transcription product of the fetal mouse skin mRNA with PCR. Then its recombinant expression vector pcDNA3.1 (-)/septin2s was constructed and used to transfect the mouse epidermal cell and fibroblast cultured in vitro. The expression of the foreign gene was detected with RT-PCR and the changes of cell proliferation were observed and analysed.RESULTS:
RT-PCR results indicated that pcDNA3.1/septin2 was expressed in the cultured mouse epidermal cells and fibroblasts in vitro. We found that the epidermal cells accelerated their reproduction, but the fibroblasts had no obvious changes.CONCLUSION:
We successfully constructed eukaryotic expressive vectors of pcDNA3.1/ septin2s and transfected it into mouse epidermal cells and fibroblasts in vitro. The results settle a basis for showing effect of septin2s on fetal mouse skin.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Recombinant
/
Transfection
/
GTP-Binding Proteins
/
Cytoskeletal Proteins
Limits:
Animals
Language:
Zh
Journal:
Zhonghua Zheng Xing Wai Ke Za Zhi
Year:
2007
Type:
Article
Affiliation country:
China