Affinity chromatography of 3 alpha-hydroxysteroid dehydrogenase from Pseudomonas testosteroni. Use of N,N-dimethylformamide to prevent hydrophobic interactions between the enzyme and the ligand.

Aukrust, L E; Norum, K R; Skalhegg, B A

Aukrust, L E; Norum, K R; Skalhegg, B A.

Biochim Biophys Acta ; 438(1): 13-22, 1976 Jun 07.

Article in En | MEDLINE | ID: mdl-181083

Subject(s)

Dimethylformamide/pharmacology; Hydroxysteroid Dehydrogenases/metabolism; Pseudomonas/enzymology; Chromatography, Affinity; Ethylenediamines; Glycocholic Acid; Hydroxysteroid Dehydrogenases/isolation & purification; NAD/pharmacology; Protein Binding; Sepharose

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Collection: 01-internacional Database: MEDLINE Main subject: Pseudomonas / Dimethylformamide / Hydroxysteroid Dehydrogenases Language: En Journal: Biochim Biophys Acta Year: 1976 Type: Article

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