Reduced topoisomerase II-mediated DNA cleavage in VP-16 resistant human leukemic cell line.
Anticancer Res
; 11(1): 445-8, 1991.
Article
in En
| MEDLINE
| ID: mdl-1850222
ABSTRACT
Drug resistance in the VP-16 resistant human leukemic cell line (THP-1/E) was studied the possible relevance of topoisomerase II activity. Strand-passing activity in crude nuclear extract from sensitive and resistant cells was comparable and equally sensitive to inhibition by VP-16. However, it was demonstrated that VP-16-mediated pBR322 DNA cleavage in the presence of nuclear extract from resistant cells was reduced to one-tenth of that from sensitive cells. These results suggested that the resistance of THP-1/E cells to VP-16 was due to reduced DNA cleavage activity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Drug Resistance
/
DNA Topoisomerases, Type II
/
Etoposide
Limits:
Humans
Language:
En
Journal:
Anticancer Res
Year:
1991
Type:
Article
Affiliation country:
Japan