Effective cleavage of structured RNAs by tandems of 10-23 DNAzymes with 3'-modified oligo(2'-O-methylribonucleotide)-effectors.
Nucleic Acids Symp Ser (Oxf)
; (52): 525-6, 2008.
Article
in En
| MEDLINE
| ID: mdl-18776485
ABSTRACT
Catalytic activity of DNAzymes targeted to IGF-I and MDR1 mRNA can be regulated by the combination of 10-23 DNAzyme with 3'-modified oligo(2'-O-methylribonucleotides) that are favorable as effectors due to their high affinity to RNA and nuclease resistance. We have demonstrated that the DNAzyme constructions designed were able to bind and cleave long structured RNA transcripts effectively under simulated physiological conditions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligoribonucleotides
/
RNA, Messenger
/
DNA, Catalytic
Language:
En
Journal:
Nucleic Acids Symp Ser (Oxf)
Year:
2008
Type:
Article
Affiliation country:
RUSSIA