Host alpha-adducin is redistributed and localized to the inclusion membrane in chlamydia- and chlamydophila-infected cells.
Microbiology (Reading)
; 154(Pt 12): 3848-3855, 2008 Dec.
Article
in En
| MEDLINE
| ID: mdl-19047752
ABSTRACT
A large-scale analysis of proteins involved in host-cell signalling pathways was performed using chlamydia-infected murine cells in order to identify host proteins that are differentially activated or localized following infection. Two proteins whose distribution was altered in Chlamydia trachomatis-infected cells relative to mock-infected cells were the actin-binding protein adducin and the regulatory kinase Raf-1. Immunoblot analysis with antibodies to both phosphorylated and non-phosphorylated forms of these proteins demonstrated that the abundance of each protein was markedly reduced in the cytosolic fraction of C. trachomatis- and Chlamydophila caviae-infected cells, but the total cellular protein abundance remained unaffected by infection. Fluorescence microscopy of chlamydia-infected cells using anti-alpha-adducin antibodies demonstrated labelling at or near the chlamydial inclusion membrane. Treatment of infected cells with nocodazole or cytochalasin D did not affect alpha-adducin that was localized to the margins of the inclusion. The demonstration of alpha-adducin and Raf-1 redistribution within cells infected by different chlamydiae provides novel opportunities for analysis of host-pathogen interactions in this system.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Calmodulin-Binding Proteins
/
Chlamydia trachomatis
/
Proto-Oncogene Proteins c-raf
/
Chlamydophila
/
Host-Pathogen Interactions
Limits:
Humans
Language:
En
Journal:
Microbiology (Reading)
Journal subject:
MICROBIOLOGIA
Year:
2008
Type:
Article
Affiliation country:
United States