Targeted transgenesis at the HPRT locus: an efficient strategy to achieve tightly controlled in vivo conditional expression with the tet system.

Palais, G; Nguyen Dinh Cat, A; Friedman, H; Panek-Huet, N; Millet, A; Tronche, F; Gellen, B; Mercadier, J-J; Peterson, A; Jaisser, F

Palais, G; Nguyen Dinh Cat, A; Friedman, H; Panek-Huet, N; Millet, A; Tronche, F; Gellen, B; Mercadier, J-J; Peterson, A; Jaisser, F.

Affiliation

Palais G; Institut National de la Santé et de la Recherche Médicale (INSERM), U772, Paris, France.

Physiol Genomics ; 37(2): 140-6, 2009 Apr 10.

Article in En | MEDLINE | ID: mdl-19141541

Subject(s)

Embryonic Stem Cells/metabolism; Hypoxanthine Phosphoribosyltransferase/genetics; Receptors, Glucocorticoid/metabolism; Tetracycline/pharmacology; Animals; Bacterial Proteins/genetics; Blotting, Western; Carrier Proteins/genetics; Cell Line; Doxycycline/pharmacology; Embryonic Stem Cells/cytology; Embryonic Stem Cells/drug effects; Female; Gene Expression Regulation/drug effects; Gene Knock-In Techniques; Genetic Vectors/genetics; Humans; Lac Operon/genetics; Liver/metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Mice, Transgenic; Myocardium/metabolism; Receptors, Glucocorticoid/genetics; Staining and Labeling; Transfection; beta-Galactosidase/genetics; beta-Galactosidase/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tetracycline / Receptors, Glucocorticoid / Embryonic Stem Cells / Hypoxanthine Phosphoribosyltransferase Limits: Animals / Female / Humans / Male Language: En Journal: Physiol Genomics Journal subject: BIOLOGIA MOLECULAR Year: 2009 Type: Article Affiliation country: France

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