[Preliminary study on purification technics of total flavonoids in Rhodiola dumulosa by macroporous resin].

ABSTRACT

OBJECTIVE: To choose the best macroporous resin for purifying total flavonoids in Rhodiola dumulosa and study various factors affecting purification of resin in order to optimize the technics. METHODS: Using methods combining static adsorption with desorption and dynamic adsorption with desorption, evaluating the technics by determination of total flavonoids in Rhodiola dumulosa using ultraviolet and visible spectrophotometry. RESULTS: The best macroporous resin was D101. The optimized technics were as follows sample total flavonoids concentration was 3.625 mg/ml, adsorption velocity was 2 BV/h, desorption ethanol concentration was 95%, desorption velocity was 2 BV/h, eluant volume was 5 BV, the purity of total flavonoids could reach 48.5%. CONCLUSIONS: Purification performance of D101 macroporous resin is the best, and it is fit for the preliminary purification of total flavonoids in Rhodiola dumulosa.