Cloning of serine protease cDNAs from Crotalus durissus terrificus venom gland and expression of a functional Gyroxin homologue in COS-7 cells.

Yonamine, C M; Prieto-da-Silva, A R B; Magalhães, G S; Rádis-Baptista, G; Morganti, L; Ambiel, F C; Chura-Chambi, R M; Yamane, T; Camillo, M A P

Yonamine, C M; Prieto-da-Silva, A R B; Magalhães, G S; Rádis-Baptista, G; Morganti, L; Ambiel, F C; Chura-Chambi, R M; Yamane, T; Camillo, M A P.

Affiliation

Yonamine CM; Centro de Biotecnologia, Instituto de Pesquisas Energéticas e Nucleares, IPEN - CNEN, Cidade Universitaria, Av. Prof Lineu Prestes 2242, CEP 05508-000, São Paulo - SP, Brazil. camilamy@usp.br

Toxicon ; 54(2): 110-20, 2009 Aug.

Article in En | MEDLINE | ID: mdl-19341755

Subject(s)

Crotalid Venoms/biosynthesis; DNA, Complementary/biosynthesis; Exocrine Glands/chemistry; Serine Endopeptidases/biosynthesis; Amino Acid Sequence; Animals; Blotting, Western; COS Cells; Chlorocebus aethiops; Cloning, Molecular; Crotalid Venoms/enzymology; Crotalid Venoms/genetics; DNA, Complementary/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/metabolism; Esterases/chemistry; Esterases/metabolism; Exocrine Glands/enzymology; Gene Library; Genetic Vectors; Mice; Molecular Weight; Plasmids/genetics; Recombinant Proteins/genetics; Serine Endopeptidases/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Serine Endopeptidases / DNA, Complementary / Crotalid Venoms / Exocrine Glands Limits: Animals Language: En Journal: Toxicon Year: 2009 Type: Article Affiliation country: Brazil

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