Comparative analyses of cell disruption methods for mitochondrial isolation in high-throughput proteomics study.

Chaiyarit, Sakdithep; Thongboonkerd, Visith

Chaiyarit, Sakdithep; Thongboonkerd, Visith.

Affiliation

Chaiyarit S; Medical Proteomics Unit, Office for Research and Development, Department of Immunology and Immunology Graduate Program, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Anal Biochem ; 394(2): 249-58, 2009 Nov 15.

Article in En | MEDLINE | ID: mdl-19622339

Subject(s)

Cell Fractionation/methods; Mitochondria/chemistry; Proteomics/methods; Animals; Cell Line; Centrifugation, Density Gradient/methods; Dogs; Freezing; Kidney/cytology; Mitochondria/metabolism; Mitochondria/ultrastructure; Mitochondrial Membranes/metabolism; Mitochondrial Proteins/analysis; Mitochondrial Proteins/metabolism; Octoxynol/metabolism; Osmolar Concentration; Polyethylene Glycols/metabolism; Proteome/analysis; Proteome/metabolism; Subcellular Fractions/chemistry; Subcellular Fractions/metabolism; Subcellular Fractions/ultrastructure; Ultrasonics/adverse effects

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Fractionation / Proteomics / Mitochondria Limits: Animals Language: En Journal: Anal Biochem Year: 2009 Type: Article Affiliation country: Thailand

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