High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases.

Demian, Douglas J; Clugston, Susan L; Foster, Meta M; Rameh, Lucia; Sarkes, Deborah; Townson, Sharon A; Yang, Lily; Zhang, Melvin; Charlton, Maura E

Demian, Douglas J; Clugston, Susan L; Foster, Meta M; Rameh, Lucia; Sarkes, Deborah; Townson, Sharon A; Yang, Lily; Zhang, Melvin; Charlton, Maura E.

Affiliation

Demian DJ; Research Technology Center, Pfizer, Inc., Cambridge, Massachusetts 02139, USA.

J Biomol Screen ; 14(7): 838-44, 2009 Aug.

Article in En | MEDLINE | ID: mdl-19641220

Subject(s)

High-Throughput Screening Assays/methods; Lipid Metabolism; Liposomes/metabolism; Phosphotransferases/metabolism; Adenosine Triphosphate/metabolism; Calorimetry; Cell-Free System; Chromatography, Thin Layer; Crystallography, X-Ray; Densitometry; High-Throughput Screening Assays/instrumentation; Humans; Inhibitory Concentration 50; Kinetics; Lipid Metabolism/drug effects; Phosphorus Radioisotopes; Phosphotransferases/antagonists & inhibitors; Protein Kinase Inhibitors/chemistry; Protein Kinase Inhibitors/pharmacology; Reproducibility of Results; Substrate Specificity/drug effects

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphotransferases / Lipid Metabolism / High-Throughput Screening Assays / Liposomes Type of study: Prognostic_studies Limits: Humans Language: En Journal: J Biomol Screen Journal subject: BIOLOGIA MOLECULAR Year: 2009 Type: Article Affiliation country: United States

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