Mast cell-mediated antigen presentation regulates CD8+ T cell effector functions.
Immunity
; 31(4): 665-76, 2009 Oct 16.
Article
in En
| MEDLINE
| ID: mdl-19818652
ABSTRACT
The characteristics, importance, and molecular requirements for interactions between mast cells (MCs) and CD8(+) T cells have not been elucidated. Here, we demonstrated that MCs induced antigen-specific CD8(+) T cell activation and proliferation. This process required direct cell contact and MHC class I-dependent antigen cross-presentation by MCs and induced the secretion of interleukin-2, interferon-gamma, and macrophage inflammatory protein-1alpha by CD8(+) T cells. MCs regulated antigen-specific CD8(+) T cell cytotoxicity by increasing granzyme B expression and by promoting CD8(+) T cell degranulation. Because MCs also upregulated their expression of costimulatory molecules (4-1BB) and released osteopontin upon direct T cell contact, MC-T cell interactions probably are bidirectional. In vivo, adoptive transfer of antigen-pulsed MCs induced MHC class I-dependent, antigen-specific CD8(+) T cell proliferation, and MCs regulated CD8(+) T cell-specific priming in experimental autoimmune encephalomyelitis. Thus, MCs are important players in antigen-specific regulation of CD8(+) T cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antigen Presentation
/
CD8-Positive T-Lymphocytes
/
Cross-Priming
/
Encephalomyelitis, Autoimmune, Experimental
/
Mast Cells
Language:
En
Journal:
Immunity
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2009
Type:
Article
Affiliation country:
Germany