Optimized procedure for expression and renaturation of recombinant human bone morphogenetic protein-2 at high protein concentrations.

Zhang, Hongbin; Wu, Jie; Zhang, Yu; Fu, Na; Wang, Jie; Zhao, Shujin

Zhang, Hongbin; Wu, Jie; Zhang, Yu; Fu, Na; Wang, Jie; Zhao, Shujin.

Affiliation

Zhang H; Department of Medical Research, Guangzhou Liuhuaqiao Hospital, 111 Liuhua Road, Guangzhou, Guangdong Province, 510010, People's Republic of China. zhangwater@tom.com

Mol Biol Rep ; 37(7): 3089-95, 2010 Oct.

Article in En | MEDLINE | ID: mdl-19823948

Subject(s)

Biochemistry/methods; Bone Morphogenetic Proteins/metabolism; Protein Renaturation; Recombinant Proteins/metabolism; Transforming Growth Factor beta/metabolism; Alkaline Phosphatase/metabolism; Animals; Blotting, Western; Bone Morphogenetic Protein 2; Bone Morphogenetic Proteins/isolation & purification; Cell Line; Fermentation; Humans; Mice; Muscles/diagnostic imaging; Muscles/pathology; Plasmids/genetics; Radiography; Rats; Rats, Sprague-Dawley; Recombinant Proteins/isolation & purification; Transforming Growth Factor beta/isolation & purification

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biochemistry / Recombinant Proteins / Transforming Growth Factor beta / Bone Morphogenetic Proteins / Protein Renaturation Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Mol Biol Rep Year: 2010 Type: Article

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