Quantum dots enhanced ultrasensitive detection of DNA adducts.
Anal Chem
; 81(24): 10285-9, 2009 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-19925008
ABSTRACT
Here we demonstrate that quantum dots (QD) can greatly improve the ultrasensitive capillary electrophoresis-laser induced fluorescence immunoassay of trace anti-benzo(a)pyrene diol epoxide (BPDE)-DNA adducts from sensitivity to separation. We for the first time show that the target QD-antibody-DNA complex is not only effectively separated but also effectively focused by capillary electrophoresis. With the online laser-induced fluorescence detection coupled, the low limits of detection of 6.6 x 10(-21) mol in mass and 120 fM in concentration are achieved for BPDE-DNA adducts. The achieved ultrasensitivity allows for human exposure biomonitoring and shows promising applications of QD in various DNA analyses, including DNA damage.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
/
DNA Adducts
/
Quantum Dots
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Anal Chem
Year:
2009
Type:
Article
Affiliation country:
China