[Pediatric osteo-articular infections with negative culture results: what about Kingella kingae?]. / Infections ostéo-articulaires septiques à culture négative chez l'enfant: avez-vous pensé à Kingella kingae?
Rev Med Suisse
; 5(224): 2235-9, 2009 Nov 04.
Article
in Fr
| MEDLINE
| ID: mdl-19994673
ABSTRACT
Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no crossreactivity with several related species and common osteoarticular pathogens. Its clinical impact is illustrated by three pediatric cases.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Diseases, Infectious
/
Neisseriaceae Infections
/
Kingella kingae
Limits:
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Language:
Fr
Journal:
Rev Med Suisse
Journal subject:
MEDICINA
Year:
2009
Type:
Article