Th17 polarized cells from nonobese diabetic mice following mycobacterial adjuvant immunotherapy delay type 1 diabetes.
J Immunol
; 184(9): 4779-88, 2010 May 01.
Article
in En
| MEDLINE
| ID: mdl-20363968
ABSTRACT
IL-17-producing T cells are regarded as potential pathogenic T cells in the induction of autoimmune diseases. Previously, we have shown that injection of adjuvants containing Mycobacterium, such as CFA or bacillus Calmette-Guérin, can prevent type 1 diabetes in NOD mice. We injected NOD mice with mycobacterial products s.c. and analyzed the IL-17-producing cells from the draining lymph nodes and spleen by restimulating whole-cell populations or CD4(+) T cells in vitro with or without IL-17-polarizing cytokines. Mice receiving CFA had a concomitant rise in the level of IL-17, IL-22, IL-10, and IFN-gamma in the draining lymph node and spleen. Adoptive transfer of splenocytes from CFA-injected NOD mice polarized with TGF-beta plus IL-6 or IL-23 delayed the development of diabetes in recipient mice. IL-17-producing cells induced by CFA maintained their IL-17-producing ability in the recipient mice. Injection of CFA also changed the cytokine profile of cells in pancreatic tissue by increasing IL-17, IL-10, and IFN-gamma cytokine gene expression. We suggest that the rise in the level of IL-17 after adjuvant therapy in NOD mice has a protective effect on type 1 diabetes development.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adjuvants, Immunologic
/
Freund's Adjuvant
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T-Lymphocytes, Helper-Inducer
/
Interleukin-17
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Diabetes Mellitus, Type 1
/
Mycobacterium tuberculosis
Limits:
Animals
Language:
En
Journal:
J Immunol
Year:
2010
Type:
Article
Affiliation country:
Canada