[Effects of Atractylodes macrocephala monosaccharide composition on cytodifferentiation and villin expression of IEC-6 cells in vitro].

ABSTRACT

OBJECTIVE: To study the influence of Atractylodes macrocephala monosaccharide composition (AMMC) on IEC-6 cells by observing cytodifferentiation and expression of villin. METHOD: Observed cell morphous under light microscope, observed cell ultramicrostructure under transmission electron microscope, evaluated villin mRNA by way of RT-PCR, and detected protein of villin by immunofluorescence. Cells were divided into the groups including blank control,positive control (given with gastrin 250 microg/L) and AMMC groups (given with AMMC 62.5, 125, 250, 500, 1000 mg/L respectively). RESULTS: 1. Cells in blank control group were immature under light microscope, which were in state of differentiation in groups with gastrin and different concentration of AMMC. 2. Cells in blank control group had bigger nucleuses with higher ratio of heterochromatin and much less villin at the edge of cells, which in groups with gastrin and different concentration of AMMC had smaller nucleuses with normal ratio of heterochromatin/heterochromatin and abundance of villin at the edge of cells. 3. Villin mRNA of IEC-6 cells in groups with gastrin and different concentration of AMMC were obviously much more than that of cells in blank control group 6 h afeer treatment. 4. Little protein of villin was detected by immunofluorescence in blank control group, which was much brighter in group with gastrin, and less bright in group with different concentration of AMMC, in which specific villin assembled. CONCLUSION: AMMC can promote cytodifferentiation by up-regulating the expression and distribution of villin in IEC-6 cells.