Identification of rickettsial infections by using cutaneous swab specimens and PCR.

Bechah, Yassina; Socolovschi, Cristina; Raoult, Didier

Bechah, Yassina; Socolovschi, Cristina; Raoult, Didier.

Affiliation

Bechah Y; Université de la Méditerranée, Marseille, France.

Emerg Infect Dis ; 17(1): 83-6, 2011 Jan.

Article in En | MEDLINE | ID: mdl-21192860

ABSTRACT

ABSTRACT

To determine the usefulness of noninvasive cutaneous swab specimens for detecting rickettsiae, we tested skin eschars from 6 guinea pigs and from 9 humans. Specimens from eschars in guinea pigs were positive for rickettsiae as long as lesions were present. Optimal storage temperature for specimens was 4 degrees C for 3 days.

Subject(s)

Rickettsia Infections/diagnosis; Rickettsia conorii/isolation & purification; Rickettsia/isolation & purification; Skin Diseases, Bacterial/diagnosis; Skin/microbiology; Specimen Handling/methods; Animals; DNA, Bacterial/analysis; DNA, Bacterial/isolation & purification; Disease Models, Animal; Guinea Pigs; Humans; Polymerase Chain Reaction/methods; Rickettsia/classification; Rickettsia/genetics; Rickettsia Infections/microbiology; Rickettsia conorii/classification; Rickettsia conorii/genetics; Skin/pathology; Skin Diseases, Bacterial/microbiology

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rickettsia / Rickettsia Infections / Skin / Specimen Handling / Skin Diseases, Bacterial / Rickettsia conorii Type of study: Diagnostic_studies / Evaluation_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Emerg Infect Dis Journal subject: DOENCAS TRANSMISSIVEIS Year: 2011 Type: Article Affiliation country: France

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