The preparation of an infectious full-length cDNA clone of Saffold virus.
Virol J
; 8: 110, 2011 Mar 09.
Article
in En
| MEDLINE
| ID: mdl-21385468
ABSTRACT
The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cloning, Molecular
/
DNA, Complementary
/
Cardiovirus Infections
/
Cardiovirus
Type of study:
Evaluation_studies
Limits:
Animals
/
Child
/
Humans
/
Male
Language:
En
Journal:
Virol J
Journal subject:
VIROLOGIA
Year:
2011
Type:
Article
Affiliation country:
Japan