Real-time PCR investigation on the expression of sboA and ituD genes in Bacillus spp.
Lett Appl Microbiol
; 52(6): 660-6, 2011 Jun.
Article
in En
| MEDLINE
| ID: mdl-21501196
ABSTRACT
AIMS:
To investigate the expression of sboA and ituD genes among strains of Bacillus spp. at different pH and temperature. METHODS ANDRESULTS:
Different Bacillus strains from the Amazon basin and Bacillus subtilis ATCC 19659 were investigated for the production of subtilosin A and iturin A by qRT-PCR, analysing sboA and ituD gene expression under different culture conditions. Amazonian strains presented a general gene expression level lower than B. subtilis ATCC 19659 for sboA. In contrast, when analysing the expression of ituD gene, the strains from the Amazon, particularly P40 and P45B, exhibited higher levels of expression. Changes in pH (6 and 8) and temperature (37 and 42 °C) caused a decrease in sboA expression, but increased ituD expression among strains from Amazonian environment.CONCLUSIONS:
Temperature and pH have an important influence on the expression of genes sboA (subtilosin A) and ituD (iturin A) among Bacillus spp. The strains P40 and P45B can be useful for the production of antimicrobial peptide iturin A. SIGNIFICANCE AND IMPACT OF THE STUDY Monitoring the expression of essential biosynthetic genes by qRT-PCR is a valuable tool for optimization of the production of antimicrobial peptides.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides, Cyclic
/
Bacillus
/
Bacillus subtilis
/
Bacteriocins
/
Gene Expression Regulation, Bacterial
/
Polymerase Chain Reaction
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Lett Appl Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2011
Type:
Article
Affiliation country:
Brazil