Differential accumulation of nif structural gene mRNA in Azotobacter vinelandii.

Hamilton, Trinity L; Jacobson, Marty; Ludwig, Marcus; Boyd, Eric S; Bryant, Donald A; Dean, Dennis R; Peters, John W

Hamilton, Trinity L; Jacobson, Marty; Ludwig, Marcus; Boyd, Eric S; Bryant, Donald A; Dean, Dennis R; Peters, John W.

Affiliation

Hamilton TL; Department of Chemistry and Biochemistry, Montana State University, Bozeman, Montana 59717, USA.

J Bacteriol ; 193(17): 4534-6, 2011 Sep.

Article in En | MEDLINE | ID: mdl-21725008

ABSTRACT

ABSTRACT

Northern analysis was employed to investigate mRNA produced by mutant strains of Azotobacter vinelandii with defined deletions in the nif structural genes and in the intergenic noncoding regions. The results indicate that intergenic RNA secondary structures effect the differential accumulation of transcripts, supporting the high Fe protein-to-MoFe protein ratio required for optimal diazotrophic growth.

Subject(s)

Azotobacter vinelandii/genetics; Bacterial Proteins/genetics; Genes, Bacterial; RNA, Messenger/genetics; Azotobacter vinelandii/growth & development; Bacterial Proteins/metabolism; Gene Expression Regulation, Bacterial; Molybdoferredoxin/chemistry; Molybdoferredoxin/genetics; Molybdoferredoxin/metabolism; Multigene Family; Mutation; Nitrogen Fixation; Nucleic Acid Conformation; RNA, Bacterial/genetics; RNA, Messenger/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / RNA, Messenger / Azotobacter vinelandii / Genes, Bacterial Language: En Journal: J Bacteriol Year: 2011 Type: Article Affiliation country: United States

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