Design and validation of consensus-degenerate hybrid oligonucleotide primers for broad and sensitive detection of corona- and toroviruses.

Zlateva, Kalina T; Crusio, Kelly M; Leontovich, Andrey M; Lauber, Chris; Claas, Eric; Kravchenko, Alexander A; Spaan, Willy J M; Gorbalenya, Alexander E

Zlateva, Kalina T; Crusio, Kelly M; Leontovich, Andrey M; Lauber, Chris; Claas, Eric; Kravchenko, Alexander A; Spaan, Willy J M; Gorbalenya, Alexander E.

Affiliation

Zlateva KT; Department of Medical Microbiology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.

J Virol Methods ; 177(2): 174-83, 2011 Nov.

Article in En | MEDLINE | ID: mdl-21864579

Subject(s)

Coronavirus/isolation & purification; DNA Primers/genetics; Reverse Transcriptase Polymerase Chain Reaction/methods; Torovirus/isolation & purification; Amino Acid Sequence; Animals; Cattle; Cell Line; Conserved Sequence; Coronavirus/classification; Coronavirus/genetics; Coronavirus Infections/diagnosis; Coronavirus Infections/virology; DNA Primers/metabolism; Humans; Molecular Sequence Data; Phylogeny; RNA, Viral/genetics; Sensitivity and Specificity; Sequence Alignment; Torovirus/classification; Torovirus/genetics; Torovirus Infections/diagnosis; Torovirus Infections/virology; Virus Cultivation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Primers / Coronavirus / Torovirus / Reverse Transcriptase Polymerase Chain Reaction Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: J Virol Methods Year: 2011 Type: Article Affiliation country: Netherlands

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