[Expressions of HIF-α and its target gene in monochorionic twin placentas with twin-twin transfusion syndrome].
Beijing Da Xue Xue Bao Yi Xue Ban
; 43(6): 792-7, 2011 Dec 18.
Article
in Zh
| MEDLINE
| ID: mdl-22178822
ABSTRACT
OBJECTIVE:
To determine hypoxia-inducible factor 1α (HIF-1α) and its target gene, vascular endothelial growth factor (VEGF) and receptor (VEGFR-1) concentrations in the placentas of the donor and recipient in monochorionic twin pregnancies with twin-twin transfusion syndrome (TTTS).METHODS:
Twenty monochorionic twin pregnancy cases were included in the study (10 with and 10 without TTTS). Tissue protein expressions of HIF-1α,VEGF and VEGFR-1 were determined by using immunohistochemistry. Western blot analysis were used to quantify and compare the protein expression. RT-PCR were used to compare their mRNA expressions.RESULTS:
HIF-1α was mainly observed in trophoblastic cells and villi capillaries endothelial cells, and VEGF in trophoblastic cells, endothelial cells and villi stromal cells; VEGFR-1 was mainly observed in villi trophoblastic cells and vascular endothelial cells. The placenta protein and mRNA expression of HIF-1α and its target gene in the donor placenta increased significantly (P<0.001) compared with that in the control placenta, but the expression of HIF-1α and its target gene in the recipients tended to be similar in the controls (P>0.05). There was no difference between the controls.CONCLUSION:
When the monochorionic twin placenta is formed in the early period, HIF-1α, VEGF and VEGFR-1 are over-expressed, which may affect the placenta angiogenesis and induce TTTS .
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Placenta
/
Vascular Endothelial Growth Factor Receptor-1
/
Vascular Endothelial Growth Factor A
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Fetofetal Transfusion
Limits:
Female
/
Humans
/
Newborn
/
Pregnancy
Language:
Zh
Journal:
Beijing Da Xue Xue Bao Yi Xue Ban
Journal subject:
MEDICINA
Year:
2011
Type:
Article
Affiliation country:
China