ChAM, a novel motif that mediates PALB2 intrinsic chromatin binding and facilitates DNA repair.
EMBO Rep
; 13(2): 135-41, 2012 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-22193777
ABSTRACT
The partner and localizer of breast cancer 2 susceptibility protein (PALB2) is crucial for the repair of DNA damage by homologous recombination. Here, we report that chromatin-association motif (ChAM), an evolutionarily conserved motif in PALB2, is necessary and sufficient to mediate its chromatin association in both unperturbed and damaged cells. ChAM is distinct from the previously described PALB2 DNA-binding regions. Deletion of ChAM decreases PALB2 and Rad51 accumulation at DNA damage sites and confers cellular hypersensitivity to the genotoxic drug mitomycin C. These results suggest that PALB2 chromatin association via ChAM facilitates PALB2 function in the cellular resistance to DNA damage.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nuclear Proteins
/
Chromatin
/
Tumor Suppressor Proteins
/
DNA Repair
Limits:
Humans
Language:
En
Journal:
EMBO Rep
Journal subject:
BIOLOGIA MOLECULAR
Year:
2012
Type:
Article
Affiliation country:
United kingdom