Delivery of MicroRNA-10b with Polylysine Nanoparticles for Inhibition of Breast Cancer Cell Wound Healing.
Breast Cancer (Auckl)
; 6: 9-19, 2012.
Article
in En
| MEDLINE
| ID: mdl-22259248
ABSTRACT
Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Breast Cancer (Auckl)
Year:
2012
Type:
Article
Affiliation country:
United States