Functional antagonism between Sall4 and Plzf defines germline progenitors.
Cell Stem Cell
; 10(3): 284-98, 2012 Mar 02.
Article
in En
| MEDLINE
| ID: mdl-22385656
ABSTRACT
Transcription factors required for formation of embryonic tissues often maintain their expression in adult stem cell populations, but whether their function remains equivalent is not clear. Here we demonstrate critical and distinct roles for Sall4 in development of embryonic germ cells and differentiation of postnatal spermatogonial progenitor cells (SPCs). In differentiating SPCs, Sall4 levels transiently increase and Sall4 physically interacts with Plzf, a transcription factor exclusively required for adult stem cell maintenance. Mechanistically, Sall4 sequesters Plzf to noncognate chromatin domains to induce expression of Kit, a target of Plzf-mediated repression required for differentiation. Plzf in turn antagonizes Sall4 function by displacing Sall4 from cognate chromatin to induce Sall1 expression. Taken together, these data suggest that transcription factors required for embryonic tissue development postnatally take on distinct roles through interaction with opposing factors, which hence define properties of the adult stem cell compartment.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cells
/
Transcription Factors
/
DNA-Binding Proteins
/
Kruppel-Like Transcription Factors
/
Germ Cells
Limits:
Animals
Language:
En
Journal:
Cell Stem Cell
Year:
2012
Type:
Article
Affiliation country:
United States