[Effect of TSA and VPA treatment on long-tailed macaque (Macaca fascicularis)-pig interspecies somatic cell nuclear transfer].
Yi Chuan
; 34(3): 342-7, 2012 Mar.
Article
in Zh
| MEDLINE
| ID: mdl-22425953
ABSTRACT
Long-tailed macaque-pig interspecies somatic cell nuclear transfer (iSCNT) is beneficial to yield embryonic stem cells from iSCNT embryos with similar genetic background as human, which can be used as materials for medical and basic research. The primary objective of this study was to investigate the effects of concentrations and treatment duration of two histone deacetylase inhibitors-Trichostatin A (TSA) and Valproic acid (VPA) and two different embryo culture media (PZM-3 and HECM-10) on the in vitro development of iSCNT embryos. The results suggested that when PZM-3 was used as the embryo culture medium, the blastocyst rate of 10 nmol/L TSA treatment for 48 h was significantly higher than the control group (22.78% vs 9.86%, P< 0.05). However, neither in PZM-3 nor in HECM-10, 2-10 mmol/L VPA treatment did not increase the in vitro developmental potential of iSCNT embryos. It was concluded that TSA treatment could enhance the in vitro developmental potential of long-tailed macaque-pig iSCNT embryos.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine
/
Valproic Acid
/
Nuclear Transfer Techniques
/
Hydroxamic Acids
/
Macaca fascicularis
Limits:
Animals
Language:
Zh
Journal:
Yi Chuan
Journal subject:
GENETICA
Year:
2012
Type:
Article
Affiliation country:
China