Expression of MMP-2, TIMP-2, TGF-ß1, and decorin in Dupuytren's contracture.
Connect Tissue Res
; 53(6): 469-77, 2012.
Article
in En
| MEDLINE
| ID: mdl-22512703
ABSTRACT
To investigate the mechanisms underlying matrix deposition in Dupuytren's disease, the expression of gelatinase A (MMP-2), the tissue inhibitor of metalloproteinase-2 (TIMP-2), transforming growth factor beta 1 (TGF-ß1), decorin (DCN), and periostin was studied. The level of relative MMP-2 activation was investigated using zymography. The mRNA expression of MMP-2, TIMP-2, TGF-ß1, and DCN was detected using reverse transcription polymerase chain reaction (RT-PCR), while the presence of protein was detected using immunohistochemical (IHC) and Western blot techniques. The level of MMP-2 activation was significantly elevated in tissues with Dupuytren's contracture. RT-PCR demonstrated significantly higher expression of MMP-2, TIMP-2, TGF-ß1, and DCN mRNA in the pathological tissues; and the IHC and immunoblotting studies revealed elevated expression of TGF-ß1, DCN, and periostin. The balance between MMP-2 and TIMP-2 was disrupted in patients with Dupuytren's disease. TGF-ß1, DCN, and periostin are involved in extracellular matrix (ECM) homeostasis in Dupuytren's contracture.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Gene Expression Regulation
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Tissue Inhibitor of Metalloproteinase-2
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Matrix Metalloproteinase 2
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Dupuytren Contracture
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Transforming Growth Factor beta1
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Decorin
Limits:
Aged
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Female
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Humans
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Male
/
Middle aged
Language:
En
Journal:
Connect Tissue Res
Year:
2012
Type:
Article
Affiliation country:
Poland