Hepatoma-derived growth factor stimulates podosome rosettes formation in NIH/3T3 cells through the activation of phosphatidylinositol 3-kinase/Akt pathway.
Biochem Biophys Res Commun
; 425(2): 169-76, 2012 Aug 24.
Article
in En
| MEDLINE
| ID: mdl-22819846
ABSTRACT
Hepatoma-derived growth factor (HDGF) stimulates the migration, invasion and metastasis in several types of cancer cells. However, the mechanism underlying HDGF-stimulated migration remains unclear. In this study, we investigated the influence of HDGF on cytoskeleton remodeling and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway in non-transformed NIH/3T3 cells. Exogenous HDGF promoted the migration and the formation of dorsal ruffles and podosome rosettes. Besides, HDGF supply increased the PI3K expression and Akt phosphorylation in dose- and time-dependent manners. Application of LY294002, a PI3K inhibitor, attenuated the HDGF-induced migration, dorsal ruffles and podosome rosettes formation. Consistently, the HDGF-overexpressing NIH/3T3 transfectants exhibited significantly increased motility and elevated PI3K/Akt activities, which were repressed by LY294002 or adenovirus-mediated overexpression of endogenous PI3K antagonist, PTEN. In summary, HDGF elicits the activation of PI3K/Akt signaling cascade, thereby promoting cytoskeleton remodeling to stimulate cellular migration.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rosette Formation
/
Intercellular Signaling Peptides and Proteins
/
Proto-Oncogene Proteins c-akt
/
Phosphatidylinositol 3-Kinase
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2012
Type:
Article