Combining bulk segregation analysis and microarrays for mapping of the pH trait in melon.
Theor Appl Genet
; 126(2): 349-58, 2013 Feb.
Article
in En
| MEDLINE
| ID: mdl-23070028
ABSTRACT
The availability of sequence information for many plants has opened the way to advanced genetic analysis in many non-model plants. Nevertheless, exploration of genetic variation on a large scale and its use as a tool for the identification of traits of interest are still rare. In this study, we combined a bulk segregation approach with our own-designed microarrays to map the pH locus that influences fruit pH in melon. Using these technologies, we identified a set of markers that are genetically linked to the pH trait. Further analysis using a set of melon cultivars demonstrated that some of these markers are tightly linked to the pH trait throughout our germplasm collection. These results validate the utility of combining microarray technology with a bulk segregation approach in mapping traits of interest in non-model plants.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biomarkers
/
Genes, Plant
/
Cucurbitaceae
/
Chromosome Segregation
/
Gene Expression Profiling
/
Polymorphism, Single Nucleotide
/
Quantitative Trait Loci
Type of study:
Prognostic_studies
Language:
En
Journal:
Theor Appl Genet
Year:
2013
Type:
Article
Affiliation country:
Israel