Natively inhibited Trypanosoma brucei cathepsin B structure determined by using an X-ray laser.
Science
; 339(6116): 227-230, 2013 Jan 11.
Article
in En
| MEDLINE
| ID: mdl-23196907
ABSTRACT
The Trypanosoma brucei cysteine protease cathepsin B (TbCatB), which is involved in host protein degradation, is a promising target to develop new treatments against sleeping sickness, a fatal disease caused by this protozoan parasite. The structure of the mature, active form of TbCatB has so far not provided sufficient information for the design of a safe and specific drug against T. brucei. By combining two recent innovations, in vivo crystallization and serial femtosecond crystallography, we obtained the room-temperature 2.1 angstrom resolution structure of the fully glycosylated precursor complex of TbCatB. The structure reveals the mechanism of native TbCatB inhibition and demonstrates that new biomolecular information can be obtained by the "diffraction-before-destruction" approach of x-ray free-electron lasers from hundreds of thousands of individual microcrystals.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trypanosoma brucei brucei
/
Cathepsin B
/
Protozoan Proteins
Limits:
Animals
Language:
En
Journal:
Science
Year:
2013
Type:
Article
Affiliation country:
Germany