Novel systems for dynamically assessing insulin action in live cells reveals heterogeneity in the insulin response.
Traffic
; 14(3): 259-73, 2013 Mar.
Article
in En
| MEDLINE
| ID: mdl-23252720
ABSTRACT
Regulated GLUT4 trafficking is a key action of insulin. Quantitative stepwise analysis of this process provides a powerful tool for pinpointing regulatory nodes that contribute to insulin regulation and insulin resistance. We describe a novel GLUT4 construct and workflow for the streamlined dissection of GLUT4 trafficking; from simple high throughput screens to high resolution analyses of individual vesicles. We reveal single cell heterogeneity in insulin action highlighting the utility of this approach - each cell displayed a unique and highly reproducible insulin response, implying that each cell is hard-wired to produce a specific output in response to a given stimulus. These data highlight that the response of a cell population to insulin is underpinned by extensive heterogeneity at the single cell level. This heterogeneity is pre-programmed within each cell and is not the result of intracellular stochastic events.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glucose Transporter Type 4
/
Insulin
Limits:
Animals
Language:
En
Journal:
Traffic
Journal subject:
FISIOLOGIA
Year:
2013
Type:
Article
Affiliation country:
Australia