A comparison of miRNA isolation and RT-qPCR technologies and their effects on quantification accuracy and repeatability.

Redshaw, Nicholas; Wilkes, Timothy; Whale, Alexandra; Cowen, Simon; Huggett, Jim; Foy, Carole A

Redshaw, Nicholas; Wilkes, Timothy; Whale, Alexandra; Cowen, Simon; Huggett, Jim; Foy, Carole A.

Affiliation

Redshaw N; LGC Limited, Queens Road, Teddington, Middlesex, UK.

Biotechniques ; 54(3): 155-64, 2013 Mar.

Article in En | MEDLINE | ID: mdl-23477383

Subject(s)

MicroRNAs/genetics; MicroRNAs/isolation & purification; Real-Time Polymerase Chain Reaction/methods; Arabidopsis/genetics; Brain Chemistry; Humans; RNA, Plant/genetics; RNA, Plant/isolation & purification; Reproducibility of Results; Sensitivity and Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: MicroRNAs / Real-Time Polymerase Chain Reaction Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Biotechniques Year: 2013 Type: Article Affiliation country: United kingdom

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