The effect of hydrogen gas on a mouse bilateral common carotid artery occlusion.
Acta Neurochir Suppl
; 118: 61-3, 2013.
Article
in En
| MEDLINE
| ID: mdl-23564105
ABSTRACT
In recent studies, molecular hydrogen selectively reduced the levels of hydroxyl radicals in vitro and exerted a therapeutic anti-oxidant activity in a rat middle cerebral artery occlusion model. The aim of this study was to investigate the effect of hydrogen gas on a mouse bilateral common carotid artery occlusion (BCCAO) model. Male C57BL/6J mice were subjected to transient BCCAO with a nontraumatic aneurysm clip. The mice were divided into three groups sham, BCCAO, and BCCAO treated with 1.3 % hydrogen gas. Cerebral blood flow (CBF) in the cortex was measured sequentially for both hemispheres with a non--invasive and noncontact laser Doppler blood perfusion imager during the procedure. Vital signs were also recorded. Oxidative stress evaluated by measuring the level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), neuronal injury in the hippocampal CA1 sector, and brain water content were assessed 24 h after ischemia. The hydrogen gas treatment had no significant effect on vital signs or CBF values. However, the reduction of the expression of 8-OHdG, the decrease in the neuronal injury in the hippocampal CA1 sector, and the attenuation in brain water content were observed in hydrogen-treated mice. In conclusion, hydrogen gas might be effective in a mouse BCCAO model.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carotid Artery, Common
/
Hydrogen
Type of study:
Diagnostic_studies
/
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Acta Neurochir Suppl
Year:
2013
Type:
Article
Affiliation country:
Japan