Validation of a cell-based ELISA as a screening tool identifying anti-alphavirus small-molecule inhibitors.

Spurgers, Kevin B; Hurt, Clarence R; Cohen, Jeffrey W; Eccelston, Lori T; Lind, Cathleen M; Lingappa, Vishwanath R; Glass, Pamela J

Spurgers, Kevin B; Hurt, Clarence R; Cohen, Jeffrey W; Eccelston, Lori T; Lind, Cathleen M; Lingappa, Vishwanath R; Glass, Pamela J.

Affiliation

Spurgers KB; US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, United States.

J Virol Methods ; 193(1): 226-31, 2013 Oct.

Article in En | MEDLINE | ID: mdl-23764417

Subject(s)

Alphavirus/drug effects; Antiviral Agents/isolation & purification; Drug Evaluation, Preclinical/methods; Animals; Antiviral Agents/pharmacology; Chlorocebus aethiops; Enzyme-Linked Immunosorbent Assay/methods; Vero Cells

Key words

50% inhibitory concentration; Alphavirus; BSA; CHIKV; CPE; Chikungunya virus; EEEV; ELISA; HRP; IC(50); MAb; MOI; PBS; RT; S/B; SD; Screen; Small-molecule inhibitor; TCID(50); TrD; Trinidad donkey strain of VEEV; USAMRIID; United States Army Medical Research Institute of Infectious Disease; VEEV; Venezuelan equine encephalitis virus; Venezuelan equine encephalitis virus (VEEV); Virus yield reduction assay; WEEV; bovine serum albumin; cytopathic effect; eastern equine encephalitis virus; enzyme-linked immunosorbent assay; horseradish peroxidase; monoclonal antibody; multiplicity of infection; pfu; phosphate-buffered saline; plaque-forming units; room temperature; signal to background ratio; standard deviation; tissue culture infectious dose 50; western equine encephalitis virus

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Antiviral Agents / Alphavirus / Drug Evaluation, Preclinical Type of study: Diagnostic_studies / Prognostic_studies / Screening_studies Limits: Animals Language: En Journal: J Virol Methods Year: 2013 Type: Article Affiliation country: United States

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