Development of a real-time PCR for detection of the oyster pathogen Nocardia crassostreae based on its homogeneous 16S-23S rRNA intergenic spacer region.

Carrasco, Noèlia; Roozenburg, Ineke; Voorbergen-Laarman, Michal; Itoh, Naoki; Engelsma, Marc Y

Carrasco, Noèlia; Roozenburg, Ineke; Voorbergen-Laarman, Michal; Itoh, Naoki; Engelsma, Marc Y.

Affiliation

Carrasco N; Laboratory for Fish and Shellfish Diseases, Central Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Electronic address: noelia.carrasco@irta.cat.

J Invertebr Pathol ; 114(2): 120-7, 2013 Oct.

Article in En | MEDLINE | ID: mdl-23876658

Subject(s)

Crassostrea/microbiology; DNA, Bacterial/analysis; Nocardia Infections/diagnosis; Nocardia/isolation & purification; RNA, Ribosomal, 16S/analysis; Real-Time Polymerase Chain Reaction/methods; Animals; DNA, Bacterial/genetics; Nocardia/genetics; RNA, Ribosomal, 16S/genetics

Key words

Crassostrea gigas; ITS; Nocardia crassostreae; Ostrea edulis; Phylogeny; Real-time PCR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / RNA, Ribosomal, 16S / Crassostrea / Real-Time Polymerase Chain Reaction / Nocardia / Nocardia Infections Type of study: Diagnostic_studies Limits: Animals Language: En Journal: J Invertebr Pathol Year: 2013 Type: Article

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