Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi.
Nature
; 501(7465): 116-20, 2013 Sep 05.
Article
in En
| MEDLINE
| ID: mdl-23913272
ABSTRACT
Newly synthesized proteins and lipids are transported across the Golgi complex via different mechanisms whose respective roles are not completely clear. We previously identified a non-vesicular intra-Golgi transport pathway for glucosylceramide (GlcCer)--the common precursor of the different series of glycosphingolipids-that is operated by the cytosolic GlcCer-transfer protein FAPP2 (also known as PLEKHA8) (ref. 1). However, the molecular determinants of the FAPP2-mediated transfer of GlcCer from the cis-Golgi to the trans-Golgi network, as well as the physiological relevance of maintaining two parallel transport pathways of GlcCer--vesicular and non-vesicular--through the Golgi, remain poorly defined. Here, using mouse and cell models, we clarify the molecular mechanisms underlying the intra-Golgi vectorial transfer of GlcCer by FAPP2 and show that GlcCer is channelled by vesicular and non-vesicular transport to two topologically distinct glycosylation tracks in the Golgi cisternae and the trans-Golgi network, respectively. Our results indicate that the transport modality across the Golgi complex is a key determinant for the glycosylation pattern of a cargo and establish a new paradigm for the branching of the glycosphingolipid synthetic pathway.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glycosylation
/
Glucosylceramides
/
Golgi Apparatus
Limits:
Animals
/
Humans
Language:
En
Journal:
Nature
Year:
2013
Type:
Article
Affiliation country:
Italy