Generation and analysis of biosensors to measure mechanical forces within cells.
Methods Mol Biol
; 1066: 169-84, 2013.
Article
in En
| MEDLINE
| ID: mdl-23955743
ABSTRACT
The inability to measure mechanical forces within cells has been limiting our understanding of how mechanical information is processed on the molecular level. In this chapter, we describe a method that allows the analysis of force propagation across distinct proteins within living cells using Förster resonance energy transfer (FRET)-based biosensors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stress, Mechanical
/
Biosensing Techniques
/
Fluorescence Resonance Energy Transfer
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2013
Type:
Article
Affiliation country:
Germany