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Recombinant antibodies to histone post-translational modifications.
Hattori, Takamitsu; Taft, Joseph M; Swist, Kalina M; Luo, Hao; Witt, Heather; Slattery, Matthew; Koide, Akiko; Ruthenburg, Alexander J; Krajewski, Krzysztof; Strahl, Brian D; White, Kevin P; Farnham, Peggy J; Zhao, Yingming; Koide, Shohei.
Affiliation
  • Hattori T; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
Nat Methods ; 10(10): 992-5, 2013 Oct.
Article in En | MEDLINE | ID: mdl-23955773
ABSTRACT
Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Histones / Protein Processing, Post-Translational / Lysine / Antibodies / Antibody Affinity Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2013 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Histones / Protein Processing, Post-Translational / Lysine / Antibodies / Antibody Affinity Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2013 Type: Article Affiliation country: United States