Transcriptome profiling of the cancer and normal tissues from gastric cancer patients by deep sequencing.
Tumour Biol
; 35(8): 7423-7, 2014 Aug.
Article
in En
| MEDLINE
| ID: mdl-24777338
ABSTRACT
Gastric cancer is the second highest cause of global cancer mortality. Genome-wide screening of transcriptome dysregulation between cancer and normal tissues would provide insights into the molecular basis of gastric cancer initiation and progression. Recently, next-generation sequencing technique has started to revolutionize biomedical studies. RNA-seq method has become a superior approach in cancer studies, which enables accurate measurement of gene expression levels. In this work, we used RNA-seq data from tumor and matched normal samples to investigate their transcriptional changes. We totally identified 114 significantly differentially expressed genes, and these genes are highly enriched in some gene ontology (GO) categories, such as "digestive system process," "regulation of body fluid levels," "secretion," "digestion," etc. This study provided the preliminary survey of the transcriptome of Chinese gastric cancer patients, which provides better insights into the complexity of regulatory changes during tumorgenesis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stomach Neoplasms
/
Sequence Analysis, RNA
/
Transcriptome
Limits:
Humans
Language:
En
Journal:
Tumour Biol
Journal subject:
NEOPLASIAS
Year:
2014
Type:
Article