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Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL.
Hirata, Kunio; Shinzawa-Itoh, Kyoko; Yano, Naomine; Takemura, Shuhei; Kato, Koji; Hatanaka, Miki; Muramoto, Kazumasa; Kawahara, Takako; Tsukihara, Tomitake; Yamashita, Eiki; Tono, Kensuke; Ueno, Go; Hikima, Takaaki; Murakami, Hironori; Inubushi, Yuichi; Yabashi, Makina; Ishikawa, Tetsuya; Yamamoto, Masaki; Ogura, Takashi; Sugimoto, Hiroshi; Shen, Jian-Ren; Yoshikawa, Shinya; Ago, Hideo.
Affiliation
  • Hirata K; 1] RIKEN SPring-8 Center, Sayo, Japan. [2] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [3].
  • Shinzawa-Itoh K; 1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
  • Yano N; 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
  • Takemura S; Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
  • Kato K; 1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
  • Hatanaka M; Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
  • Muramoto K; Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
  • Kawahara T; Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
  • Tsukihara T; 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [3] Institute for Protein Research, Osaka University, Suita, Japan.
  • Yamashita E; Institute for Protein Research, Osaka University, Suita, Japan.
  • Tono K; Japan Synchrotron Radiation Research Institute, Sayo, Japan.
  • Ueno G; RIKEN SPring-8 Center, Sayo, Japan.
  • Hikima T; RIKEN SPring-8 Center, Sayo, Japan.
  • Murakami H; RIKEN SPring-8 Center, Sayo, Japan.
  • Inubushi Y; RIKEN SPring-8 Center, Sayo, Japan.
  • Yabashi M; RIKEN SPring-8 Center, Sayo, Japan.
  • Ishikawa T; RIKEN SPring-8 Center, Sayo, Japan.
  • Yamamoto M; RIKEN SPring-8 Center, Sayo, Japan.
  • Ogura T; Picobiology Institute, Graduate School of Life Science, University of Hyogo, Sayo, Japan.
  • Sugimoto H; RIKEN SPring-8 Center, Sayo, Japan.
  • Shen JR; Photosynthesis Research Center, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
  • Yoshikawa S; Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
  • Ago H; RIKEN SPring-8 Center, Sayo, Japan.
Nat Methods ; 11(7): 734-6, 2014 Jul.
Article in En | MEDLINE | ID: mdl-24813624
ABSTRACT
We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Electron Transport Complex IV / Crystallography / Lasers Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2014 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Electron Transport Complex IV / Crystallography / Lasers Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2014 Type: Article