Determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma by column switching HPLC.
J Pharm Biomed Anal
; 7(12): 1765-76, 1989.
Article
in En
| MEDLINE
| ID: mdl-2490565
ABSTRACT
A HPLC method for the determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma is reported. The metabolites under investigation were the N4-acetyl sulfonamides and 3'- and 4'-demethyl trimethoprim. In order to obtain a sensitivity of 25-50 ng ml-1, the application of column switching HPLC was investigated. An on-line preconcentration of the drugs and metabolites was preceded by an off-line sample pre-treatment. Parent compounds and metabolites were separated by reversed-phase HPLC followed by UV-detection. The mean recoveries for 4'-demethyl trimethoprim were greater than 80% while the mean recoveries for the other compounds were greater than 90%. Application of the method for analysis of plasma samples obtained from pharmacokinetic studies is described.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfadimethoxine
/
Sulfamethoxazole
/
Trimethoprim
Limits:
Animals
Language:
En
Journal:
J Pharm Biomed Anal
Year:
1989
Type:
Article
Affiliation country:
Netherlands