Development of a multiplex assay for genus- and species-specific detection of Phytophthora based on differences in mitochondrial gene order.

Bilodeau, Guillaume J; Martin, Frank N; Coffey, Michael D; Blomquist, Cheryl L

Bilodeau, Guillaume J; Martin, Frank N; Coffey, Michael D; Blomquist, Cheryl L.

Phytopathology ; 104(7): 733-48, 2014 Jul.

Article in En | MEDLINE | ID: mdl-24915428

Subject(s)

Multiplex Polymerase Chain Reaction/methods; Phytophthora/isolation & purification; Plant Diseases/parasitology; DNA Primers/genetics; DNA, Intergenic/chemistry; DNA, Intergenic/genetics; DNA, Mitochondrial/chemistry; DNA, Mitochondrial/genetics; DNA, Ribosomal Spacer/chemistry; DNA, Ribosomal Spacer/genetics; Genetic Markers/genetics; Phylogeny; Phytophthora/classification; Phytophthora/genetics; Plants/parasitology; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Sequence Analysis, DNA; Species Specificity

Key words

real-time PCR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phytophthora / Plant Diseases / Multiplex Polymerase Chain Reaction Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: Phytopathology Journal subject: BOTANICA Year: 2014 Type: Article

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