Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development.
Dev Biol
; 393(1): 71-83, 2014 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-24984259
ABSTRACT
E-twenty six variant 2 (Etv2) transcription factor participates in cardiac, vascular-endothelial and blood cell lineage specification decisions during embryonic development. Previous studies have identified genomic elements in the etv2 locus responsible for vascular endothelial cell specification. Using transgenic analysis in zebrafish, we report here an etv2 proximal promoter fragment that prevents transgene misexpression in myocardial progenitor cells. This inhibition of etv2 expression in the cardiac progenitor population is partly mediated by Scl and Nkx2.5, likely through direct binding to the etv2 promoter, and cis-regulatory elements located in the first and second introns. The results identify an etv2 cis-regulatory mechanism controlling cardiovascular fate choice implying that etv2 participates in a transcriptional network mediating developmental plasticity of endothelial progenitor cells during embryonic development.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
Zebrafish
/
Endothelium, Vascular
/
Zebrafish Proteins
/
Heart
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Dev Biol
Year:
2014
Type:
Article
Affiliation country:
United States