[Synergism of bromodeoxyuridine and mitomycin C in the production of chromosomal aberrations in Fanconi's anemia]. / Sinergismo bromodesoxiuridina-mitomicina C en la produccion de aberraciones cromósomicas en anemia de Fanconi.

ABSTRACT

Synergism of BrdU and MMC on the production of chromosome aberrations in lymphocytes of patients with Fanconi's anemia (FA) was studied. With this purpose, the lymphocytes of an FA patient and of a normal subject were cultured and exposed to BrdU and to MMC in 6 different experiments. The results showed no synergism of these two agents in normal cells. However FA cells exposed to BrdU and MMC showed an increased frequency of aberrations suggesting synergism. Furthermore the chromosome damage produced by MMC was significantly increased by the presence of BrdU in the medium. These results support the hypothesis of a defect of DNA repair in FA cells.