Acute and perinatal programming effects of a fat-rich diet on rat muscle mitochondrial function and hepatic lipid accumulation.
Acta Obstet Gynecol Scand
; 93(11): 1170-80, 2014 Nov.
Article
in En
| MEDLINE
| ID: mdl-25052904
ABSTRACT
OBJECTIVE:
Maternal high-fat intake during pregnancy may have long-term consequences in the offspring. Since this might relate to the capacity of mitochondrial metabolic adaptation and hepatic lipid metabolism, we investigated how maternal high-fat intake affected mitochondrial function and hepatic steatosis in the offspring.DESIGN:
Sprague-Dawley rats were fed a high-fat (20% w/w) or a control diet (chow, C) from 10 days before pregnancy and throughout lactation. At weaning the litters were split into two groups; one was continued on the maternal diet and the other was fed low-fat chow. SAMPLE Skeletal muscle mitochondria and liver lipids.METHODS:
Mitochondrial respiration and hepatic lipid content were determined during and after weaning, on days 20 and 70 postpartum. MAIN OUTCOMEMEASURES:
Mitochondrial function and hepatic lipids.RESULTS:
At 20 days, maternal high-fat diet caused increased Vo2max with pyruvate as substrate (p=0.047), at 70 days, pups born by C-dams, but not those born by high-fat-dams, showed increased oxidation of palmitoylcarnitine in the absence of ADP (p=0.018). Rates of ADP-stimulated oxygen consumption, maximal respiratory capacity and mitochondrial respiratory control ratio with pyruvate, increased post weaning (p<0.001), whereas respiratory control ratio with palmitoylcarnitine decreased (p=0.013). The increase in respiratory control ratio was most pronounced in pups from C-dams (p=0.05). The high-fat-diet caused pronounced hepatic steatosis in pups at weaning (p<0.001), without concomitant ceramide accumulation, while high-fat-feeding after weaning induced triacylglycerol and ceramide accumulation (p<0.01), regardless of maternal diet.CONCLUSION:
Intake of a fat-rich diet during pregnancy and lactation reduced the age-induced increases in un-coupled fat oxidation.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prenatal Exposure Delayed Effects
/
Fetal Development
/
Lipid Metabolism
/
Fatty Liver
/
Diet, High-Fat
/
Mitochondria, Muscle
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Acta Obstet Gynecol Scand
Year:
2014
Type:
Article
Affiliation country:
Denmark