Complete mitochondrial genome of Paramisgurnus dabryanus.
Mitochondrial DNA A DNA Mapp Seq Anal
; 27(2): 1257-9, 2016.
Article
in En
| MEDLINE
| ID: mdl-25090380
ABSTRACT
In this study, the complete mitochondrial genome of Paramisgurnus dabryanus was obtained by PCR base on 18 pairs of primers. Among the 18 primers, the 14 primers were from the previously published universal primers for Cyprinus carpio L. mitogenome amplification. The remaining 4 primers were designed on the basis of related species mtDNA sequences. The genome is 16,570 bp in length, including 2 ribosomal RNA genes. 13 proteins-coding genes, 22 transfer RNA genes, and a non-coding control region, the gene composition and order of which was similar to most reported from other vertebrates. Sequence analysis showed that the overall base composition of Paramisgurnus dabryanus is T 27.3%, C 26.9%, A 28.5%, and G 17.4%. The sequence is a slight A + T bias of 55.8%, which is similar to other fishes. Mitochondrial genome is widely used in phylogenetic analysis, evolutionary genomics, species identification and related research of fish.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cypriniformes
/
DNA, Mitochondrial
/
Sequence Analysis, DNA
/
Genome, Mitochondrial
/
Mitochondria
Limits:
Animals
Language:
En
Journal:
Mitochondrial DNA A DNA Mapp Seq Anal
Year:
2016
Type:
Article