Stacking and analysis of melamine in milk products with acetonitrile-salt stacking technique in capillary electrophoresis.
J Anal Methods Chem
; 2014: 212697, 2014.
Article
in En
| MEDLINE
| ID: mdl-25197612
ABSTRACT
Melamine was measured in real milk products with capillary electrophoresis (CE) based on acetonitrile-salt stacking (ASS) method. Real milk samples were deproteinized with acetonitrile at a final concentration of 60% (v/v) and then injected hydrodynamically at 50 mBar for 40.0 s. The optimized buffer contains 80.0 mmol/L pH 2.8 phosphates. Melamine could be detected within 20.0 min at +10 kV with a low limit of detection (LOD) of 0.03 µmol/L. Satisfactory reproducibility (inter- and intraday RSD% both for migration time and peak area was lower than 5.0%) and a wide linearity range of 0.05 µmol/L ~ 10.0 µmol/L were achieved. The proposed method was suitable for routine assay of MEL in real milk samples that was subjected to a simple treatment step.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Anal Methods Chem
Year:
2014
Type:
Article
Affiliation country:
China